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aml 193 cell line  (DSMZ)


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    DSMZ aml 193 cell line
    Aml 193 Cell Line, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aml+193+cell+line/pm37568827-70-1-27?v=DSMZ
    Average 93 stars, based on 23 article reviews
    aml 193 cell line - by Bioz Stars, 2026-08
    93/100 stars

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    Evaluation of the STING pathway across common AML cell lines. A, Indicated AML cell lines plated 1 × 10 5 cells/well were stimulated 72 hours with CDNs at 10 µg/mL, and supernatants were harvested for ELISA ( n = 5). An assay detection limit of 50 pg/mL is shown. qPCR analysis ( B ) and immunoblot ( C ) of STING pathway and resistance genes across AML lines ( n = 1). For qPCR, data represent GAPDH-normalized fold expression relative to normal CD11b + PBMC controls.

    Journal: Cancer Research Communications

    Article Title: Identification of Nonfunctional Alternatively Spliced Isoforms of STING in Human Acute Myeloid Leukemia

    doi: 10.1158/2767-9764.CRC-24-0095

    Figure Lengend Snippet: Evaluation of the STING pathway across common AML cell lines. A, Indicated AML cell lines plated 1 × 10 5 cells/well were stimulated 72 hours with CDNs at 10 µg/mL, and supernatants were harvested for ELISA ( n = 5). An assay detection limit of 50 pg/mL is shown. qPCR analysis ( B ) and immunoblot ( C ) of STING pathway and resistance genes across AML lines ( n = 1). For qPCR, data represent GAPDH-normalized fold expression relative to normal CD11b + PBMC controls.

    Article Snippet: Human AML cell lines were purchased from ATCC (Kasumi-1, Mv-411, AML193, HL-60) or DSMZ (U937, OCI-AML3) all in 2017–2018.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

    Figure 1. The expression of lncRNA AL645608.3 in various AML cells. A. CCK-8 for detecting the cell viability of THP- 1, HL-60, KG-1, and AML-193 cell lines. B. RT-qPCR for measuring the expression of AL645608.3 in the above cell lines. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Journal: American Journal of Translational Research

    Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

    doi: 10.62347/txka6586

    Figure Lengend Snippet: Figure 1. The expression of lncRNA AL645608.3 in various AML cells. A. CCK-8 for detecting the cell viability of THP- 1, HL-60, KG-1, and AML-193 cell lines. B. RT-qPCR for measuring the expression of AL645608.3 in the above cell lines. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Article Snippet: AML cell lines (THP-1, HL-60, KG-1, and AML193) purchased from the American Type Culture Collection (Manassas, USA) were cultivated in RPMI 1640 medium (#SH30809.01; Hyclone, Utah, USA) with fetal bovine serum (FBS; #141215; Hangzhou Tianhang Biotechnology Co., LTD., Zhejiang, China).

    Techniques: Expressing, CCK-8 Assay, Quantitative RT-PCR

    Figure 4. Expression of IRF6 and CBL in diverse AML cells. A. RT-qPCR for detection of the mRNA level of IRF6 in THP-1, HL-60, KG-1, and AML-193 cell lines. B. RT-qPCR for measuring the mRNA level of CBL in the above AML cells. C-E. Western blot of the protein levels of IRF6 and CBL in the above AML cells. ns, P>0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Journal: American Journal of Translational Research

    Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

    doi: 10.62347/txka6586

    Figure Lengend Snippet: Figure 4. Expression of IRF6 and CBL in diverse AML cells. A. RT-qPCR for detection of the mRNA level of IRF6 in THP-1, HL-60, KG-1, and AML-193 cell lines. B. RT-qPCR for measuring the mRNA level of CBL in the above AML cells. C-E. Western blot of the protein levels of IRF6 and CBL in the above AML cells. ns, P>0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Article Snippet: AML cell lines (THP-1, HL-60, KG-1, and AML193) purchased from the American Type Culture Collection (Manassas, USA) were cultivated in RPMI 1640 medium (#SH30809.01; Hyclone, Utah, USA) with fetal bovine serum (FBS; #141215; Hangzhou Tianhang Biotechnology Co., LTD., Zhejiang, China).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Figure 5. Effects of AL645608.3 on the mRNA expression of IRF6, CBL and IFNB1 in AML cells. A-C. The mRNA expression of IRF6, CBL and IFNB1 in THP-1 cells infected with sh-AL645608.3 lentiviruses. D-F. The mRNA expres sion of IRF6, CBL and IFNB1 in AML-193 cells infected with AL645608.3 overexpression lentiviruses. ns, P>0.05; ****P<0.0001.

    Journal: American Journal of Translational Research

    Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

    doi: 10.62347/txka6586

    Figure Lengend Snippet: Figure 5. Effects of AL645608.3 on the mRNA expression of IRF6, CBL and IFNB1 in AML cells. A-C. The mRNA expression of IRF6, CBL and IFNB1 in THP-1 cells infected with sh-AL645608.3 lentiviruses. D-F. The mRNA expres sion of IRF6, CBL and IFNB1 in AML-193 cells infected with AL645608.3 overexpression lentiviruses. ns, P>0.05; ****P<0.0001.

    Article Snippet: AML cell lines (THP-1, HL-60, KG-1, and AML193) purchased from the American Type Culture Collection (Manassas, USA) were cultivated in RPMI 1640 medium (#SH30809.01; Hyclone, Utah, USA) with fetal bovine serum (FBS; #141215; Hangzhou Tianhang Biotechnology Co., LTD., Zhejiang, China).

    Techniques: Expressing, Infection, Over Expression

    Figure 6. Effects of AL645608.3 on the protein expression of IRF6, CBL and IFNB1 in AML cells. A. Representa tive western blot images for IRF6, CBL and IFNB1 in THP-1 cells with sh-AL645608.3 lentiviruses infection and in AML-193 cells with AL645608.3 overexpression lentiviruses infection. B-D. Western blot for the detection of protein levels of IRF6, CBL and IFNB1 in THP-1 cells infected with sh-AL645608.3 lentiviruses. E-G. Western blot for the detection of protein levels of IRF6, CBL and IFNB1 in AML-193 cells infected with AL645608.3 overexpression len tiviruses. ns, P>0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Journal: American Journal of Translational Research

    Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

    doi: 10.62347/txka6586

    Figure Lengend Snippet: Figure 6. Effects of AL645608.3 on the protein expression of IRF6, CBL and IFNB1 in AML cells. A. Representa tive western blot images for IRF6, CBL and IFNB1 in THP-1 cells with sh-AL645608.3 lentiviruses infection and in AML-193 cells with AL645608.3 overexpression lentiviruses infection. B-D. Western blot for the detection of protein levels of IRF6, CBL and IFNB1 in THP-1 cells infected with sh-AL645608.3 lentiviruses. E-G. Western blot for the detection of protein levels of IRF6, CBL and IFNB1 in AML-193 cells infected with AL645608.3 overexpression len tiviruses. ns, P>0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Article Snippet: AML cell lines (THP-1, HL-60, KG-1, and AML193) purchased from the American Type Culture Collection (Manassas, USA) were cultivated in RPMI 1640 medium (#SH30809.01; Hyclone, Utah, USA) with fetal bovine serum (FBS; #141215; Hangzhou Tianhang Biotechnology Co., LTD., Zhejiang, China).

    Techniques: Expressing, Western Blot, Infection, Over Expression

    Figure 7. AL645608.3 directly enhanced IRF6 expression in AML cells. A. Co-IP for IRF6 in THP-1 cells with infection of sh-AL645608.3 lentiviruses as well as AML-193 cells with infection of AL645608.3 overexpression lentiviruses. B-E. Quantification of IRF6 and ubiquitin expression in above THP-1 and AML-193 cells. ns, P>0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Journal: American Journal of Translational Research

    Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

    doi: 10.62347/txka6586

    Figure Lengend Snippet: Figure 7. AL645608.3 directly enhanced IRF6 expression in AML cells. A. Co-IP for IRF6 in THP-1 cells with infection of sh-AL645608.3 lentiviruses as well as AML-193 cells with infection of AL645608.3 overexpression lentiviruses. B-E. Quantification of IRF6 and ubiquitin expression in above THP-1 and AML-193 cells. ns, P>0.05; **P<0.01; ***P<0.001; ****P<0.0001.

    Article Snippet: AML cell lines (THP-1, HL-60, KG-1, and AML193) purchased from the American Type Culture Collection (Manassas, USA) were cultivated in RPMI 1640 medium (#SH30809.01; Hyclone, Utah, USA) with fetal bovine serum (FBS; #141215; Hangzhou Tianhang Biotechnology Co., LTD., Zhejiang, China).

    Techniques: Expressing, Co-Immunoprecipitation Assay, Infection, Over Expression, Ubiquitin Proteomics

    Figure 8. MMP-9 was activated in AML cells with AL645608.3 overexpression. A, B. Gelatin zymography assay for MMP-9 in THP-1, HL-60, KG-1, and AML-193 cell lines. C-E. Gelatin zymography assay for MMP-9 in THP-1 cells with infection of sh-AL645608.3 lentiviruses and AML-193 cells with infection of AL645608.3 overexpression lentivi ruses. ns, P>0.05; ****P<0.0001.

    Journal: American Journal of Translational Research

    Article Title: LncRNA AL645608.3 mediates malignant progression of acute myeloid leukemia

    doi: 10.62347/txka6586

    Figure Lengend Snippet: Figure 8. MMP-9 was activated in AML cells with AL645608.3 overexpression. A, B. Gelatin zymography assay for MMP-9 in THP-1, HL-60, KG-1, and AML-193 cell lines. C-E. Gelatin zymography assay for MMP-9 in THP-1 cells with infection of sh-AL645608.3 lentiviruses and AML-193 cells with infection of AL645608.3 overexpression lentivi ruses. ns, P>0.05; ****P<0.0001.

    Article Snippet: AML cell lines (THP-1, HL-60, KG-1, and AML193) purchased from the American Type Culture Collection (Manassas, USA) were cultivated in RPMI 1640 medium (#SH30809.01; Hyclone, Utah, USA) with fetal bovine serum (FBS; #141215; Hangzhou Tianhang Biotechnology Co., LTD., Zhejiang, China).

    Techniques: Over Expression, Zymography Assay, Infection